what happens if you swallow chewing tobacco spit Is It Safe to Snuff Tobacco? Health Risks and Potential Concerns What Happens if you Swallow

Abbreviations MSCs: Mesenchymal stem cells DPSCs: Dental pulp stem cells IC50: Half maximal inhibitory concentration E-cigs: Electronic cigarettes ROS: Reactive oxygen species FBS: Fetal bovine serum TE-p: Tobacco extract powder e-Cig-LV: Electronic cigarette liquid vape ALP: Alkaline phosphatase RANKL: Receptor activator of nuclear factor kappa- ligand ARS: Alizarin red stain References Biehl JK, Russell B (2009) Introduction to stem cell therapy

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Material and methods Cell culture Human epithelial cancer cell lines from different head and neck regions UM-SCC-1 (floor of mouth), WSU-HN6 (tongue), and WSU-HN30 (pharyngeal) were cultured in Dulbeccos modified Eagles Medium (DMEM) supplemented with 10% fetal bovine serum, 1% L-glutamine, and 1% antibioticantimycotic (Gibco)

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6 : 461471 Delclos K, Kadlubar F