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Briefly, cigarette smoke from one cigarette was collected from the side of the combustion zone with neither puffing nor passing through a filter, and bubbled into 10 mL of sterile PBS in a 50 mL centrifuge tube at minimum speed using a suction pump (Tokyo M.I., Tokyo, Japan, Cat# M20)

Trends in Cigarette Consumption Within Subgroups of Smokers Across the study period, mean overall cigarette consumption was consistently higher among daily smokers and those who were older, men, from more disadvantaged social grades (C2DE), and living in the north (vs

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However, subsequent studies did not confirm this correlation [29]