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Peak pairs with matching exact mass and retention time were identified and flagged as potential isotopologues using an exact mass difference of 200 ppm, a retention time difference of 0.4 min, a Pearson correlation coefficient between the peak area of the predicted isotopologue and its parent across samples 0.7 and a requirement that the peak area of the predicted isotopologue be less than that of its parent

In addition to hAEC itself, secreted products derived from these cells exerted similar cytotoxic and anti-proliferative effects against cancer cell lines in vitro