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For the recordings, slices were transferred to a recording chamber continuously perfused with aCSF and visualized on an upright microscope (Zeiss, Germany) using a 60x water immersion objective, infrared differential interference contrast optics, and a CMOS camera (Hamamatsu, Japan)

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To advance our understanding, it is essential that future research integrates biochemical characterization of each variants impact on enzyme function with in vivo modeling of physiological outcomes

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doi: 10.1002/jmri.26046