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doi: 10.1152/ajprenal.00285.2002 47 International Agency for Research on Cancer (2012)

Fujino, G

Dock5 deficiency promotes proteinuric kidney diseases via modulating podocyte lipid metabolism

Animals A total of 37 male Wistar rats (810 weeks old, 250350 g) were purchased from the Multidisciplinary Center for Biological Investigation (CEMIB), University of Campinas (UNICAMP), Campinas, SP, Brazil, and housed at the Multidisciplinary Animal Experimentation Unit (UMEA), Faculty of Medical Sciences (FCM), UNICAMP, in groups of three animals per cage with free access to standard feed and water

Protein and lipid pellets were discarded, while 10 L injections of supernatants were analyzed via a Vanquish UHPLC system (Thermo Fisher Scientific) coupled to a Q Exactive mass spectrometer (Thermo Fisher Scientific) utilizing an untargeted acquisition as previously reported 18