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Seeds were surface sterilised using chlorine gas for 4 h, and then stratified for at least 3 days at 4 C in water
At least one study has shown vitamin C to have a half-life of about two hours, meaning the amount present in the body reduces every two hours

The rapid production of ROS upon activation is termed oxidative burst, which is catalysed by a multicomponent enzyme, NADPH oxidase (NOX) 7
The abnormally low plasma selenium levels in peptic ulcer patients may be another major cause for the poor blood GPx activity in those patients [58]

doi: 10.3389/fimmu.2018.00754
Finally, the sections were counterstained with hematoxylin for 20 seconds and dehydrated
