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MR analyses confirm a causal relationship between gut dysbiosis and periodontitis, highlighting their interconnected pathogenesis

Each sample (8 l) was injected onto an Agilent Poroshell 120 EC-C18 (100 2.1 mm, 2.7 um) in a Shimadzu Nexera UHPLC system at a flowrate of 0.2 ml/min using a gradient elution created between buffer A (10 mM tributylamine aqueous solution adjusted pH to 4.95 with 15 mM acetic acid) and buffer B of methanol, from 10% to 40% (buffer B) in 30 min at room temperature

Venkataratnam Y, Mukherjee V, Rai N, Mahalingaswamy M, Shandilya D, Konar S, Nanjegowda NB, Waghmare G, Nanjaiah ND

Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta C(T)) method

Morelli MB, Gambardella J, Castellanos V, Trimarco V, Santulli G
