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Subsequently, the cells were washed with PBS and blocked for 1 h with 1% bovine serum albumin (BSA, Sigma A9418) and 22.52 mg/mL glycine in PBST (PBS + 0.05% Tween-20, LPS Solution CBP007T)

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Unlike plain sterile water, which must be used immediately after opening due to contamination risk, bacteriostatic water is designed for multi-dose use, the benzyl alcohol inhibits microbial growth, allowing the vial to be accessed multiple times over a 28-day period while maintaining sterility