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A 3D spheroid model of primary human hepatocytes revealed that Wnt/catenin signaling induces major hepatocyte proliferation by suppressing the p53PAI1 signaling axis

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ROS and glutathione measurement For ROS measurement, T cells were incubated at 37 C for 20 min in PBS containing DCFHDA (1:1000, #S0033S, Beyotime, China) for cellular ROS detection or with Mito-SOX (1:200, #M36008, Invitrogen, USA) for mitochondrial ROS detection

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Administering the Fat Dissolving Injection After a 20-minute waiting period, I proceeded with the injections

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