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Revised Date: January 18, 2023 Recommendations and Topics Scope Key Recommendations Background Epidemiology Patient Risk Factors Associated with B 12 Deficiency Patient Risk Factors Associated with Folate Deficiency Prevention Diagnosis Management Ongoing Care References Resources Appendix A: Dietary sources of cobalamin Scope This guideline covers the primary care investigation and management of cobalamin (vitamin B 12 or simply B 12 ) and folate deficiency in adults

Model B was adjusted for the associates of the studied deficiencies which we considered to be confounders since most of them differed between the participants based on their deficiency status and are directly or indirectly related to the deficiencies and the outcome [12, 14, 24]

(3) Ultrasonic extraction in an ice-water bath for 10 min, and leave at -40C overnight

2 References 1 Weiss, Jessica B., et al

Synthetic peptides provide cost efficiency and scalability, while biotechnological approaches offer superior purity and sustainability