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Primary antibody incubations were followed by signal detection using BOND Polymer Refine Detection Kit (Leica Biosystems, IL, USA) with Mayers hematoxylin nuclear counter stain as per manufacturers instructions

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In 2013, Camenisch et al

Copper is an essential trace element, but excess copper is toxic
Int J Med Microbiol 290, 491496, doi: 10.1016/S1438-4221(00)80073-9 (2000)
