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Thus, heterogeneity of m should be included in the context of tumor heterogeneity and considered a factor to analyze when interpreting changes induced for genetic modifications or anticancer therapies that directly target or influence mitochondrial metabolism ( Because of the above-described considerations, it is of high importance to standardize dye loading concentration, loading time, temperature and utilize comparable methods to quantitatively analyze m ( 3.2.1 Fluorophores commonly used to determine mitochondrial membrane potential Available mitochondrial membrane potentiometric dyes for fluorescent imaging include tetramethyl rhodamine methyl ester (TMRM, Figure 3 ) and ethyl ester (TMRE), Rhodamine 123 (Rho123), and the ratiometric dye 5,5,6,6-tetrachloro-1,1,3,3-tetraethylbenzimidazolyl-carbocyanine iodide (JC-1) ( Table 2 )

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