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Next day, the sections were washed three times in PBS followed by incubation with Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (A-11008, Invitrogen) and Alexa Fluor 594 anti-mouse CD68 Antibody (137020, BioLegend, USA) or Alexa Fluor 594 anti-mouse Ly-6G Antibody (127636, BioLegend, USA) for 1 h at room temperature in the dark followed by washing and mounting

Borna Vrdoljak, Departments of Pharmacology and Pathology, Medical Faculty University of Zagreb, Zagreb 10000, Croatia

For research use only

doi: 10.1186/s12935-023-03052-z

Common adverse effects: Injection site reactions: Pain, redness, swelling, or bruising at the injection site are frequently reported

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