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glutathione levels and immune responses in tuberculosis patients Immunity to TB | Immunopaedia
Description
This procedure involved removing the supernatant and resuspending the mitochondrial pellet in an isolation buffer of 250 mM sucrose, 0.5 mM EDTA, and 10 mM Tris-HCl to achieve a final protein concentration of 20-25 mg/ml [2]

It acts as a chemoattractant, recruiting immune cells like macrophages and mast cells to the site of investigation

10.1016/j.jid.2023.03.1408 23 KimC

10.1074/jbc.r110.122218 J

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the injection instructions were also simple to follow
