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Mitochondrial dysfunction, particularly in hyperglycemic conditions, results in excess ROS due to impaired electron transfer, which promotes superoxide production [20]

Activated calpains can aberrantly cleave several proteins that are critical for the integrity of neurons, such as spectrin, tubulin, tau, microtubule-associated protein, and neurofilaments (Wang and Po-Wai, 1994)

Next, the sections were washed and counterstained with DAPI for 8 min in the dark and mounted using mounting medium

Neuroimmunomodulation by Macrophage Migration Inhibitory Factor (MIF)