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Introduction Diabetes mellitus (DM) is a multifactorial metabolic disorder characterized by chronic hyperglycemia, which is caused due to impaired insulin secretion, insulin resistance, or both

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When the column is centrifuged (Mini: 12-14,000 x g for 1 min, Midi: 750-1,500 x g for 5 min) the pores of the membrane dilate (0.1-0.2 m) and allow the sample to be purified and collected for subsequent downstream analyses