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The first major hurdle that became apparent shortly after the first IGF-1 assay was the discovery of IGFBPs, the presence of which significantly reduced the levels of immunoreactive IGF-1 in samples leading to misleadingly low results

Scannell, J

J Biol Chem 277(19):1685316859

Expression profiles of GPR21, GPR39, GPR135, and GPR153 orphan receptors in different cancers

Variables significant in the univariate analysis were then included in the multivariate model to assess their independent impact on survival after adjusting for other covariates