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Down-regulation of TMAO levels by dimethyl-1-butanol (DMB), a TMA formation inhibitor, is able to ameliorate the cognitive deterioration and long-term potentiation (LTP) in APP/PS1 mice and decrease amyloid- (A)1-42, -secretase, and -secretase-cleaved C-terminal fragment (CTF) levels in the hippocampus (Morello et al., 2000)

For HCV subtyping by phylogenetic analysis, nucleotide sequences from reference HCV isolates from each genotype, subtype and clade were retrieved from the HCV Los Alamos Database ( and International Committee on Taxonomy of Viruses ( and were aligned with the Portuguese sequences using MAFFT

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Competing interests: The authors declare no conflicts of interest