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Abstract Glioblastoma remains the most aggressive and treatment-resistant brain malignancy, driven by genetic heterogeneity, metabolic plasticity, and an immunosuppressive tumor microenvironment (TME)

Recently, genome-wide CRISPR/Cas9-mediated loss-of-function screens showed that loss of TP53, BAX or PMAIP1 renders AML cells resistant to Venetoclax 20

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Its use during screening has many advantages, as it reduces the use of laboratory animals, it is more cost-effective than the mammalian in vivo studies, results are obtained fast due to the short organogenesis period (5.25 h post fertilization (hpf) until 120 hpf) and only a small amount of test compound is needed due to the small size of the embryos