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MitoSOX Red indicator and PI were detected using the TRITC filter (Ex/Em: 396/610 nm) and Texas Red (Ex/Em:560/630), respectively, while Hoechst was detected using HOECHST 33342 filter (Ex/Em: 353/483)

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These farmers should consider the impact of less vitamin B12/cobalt and selenium going into their animals and revisit their supplementation plan to ensure growth is not limited by a mineral deficiency.