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Some of the most common thermo-responsive polymers used to prepare IN gel formulations include poloxamers (poloxamer 188 and poloxamer 407) and chitosan [26,27]

Following treatment medium was replaced and cell viability was assessed after 16 h (Naisbitt et al., 1998) by the resazurin reduction assay as described before (Sison-Young et al., 2017)