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BT474 cells and PC3- hTERT cells were cultured in DMEM F-12 medium (Gibco, Invitrogen) with 10% FBS (Gibco, Invitrogen) and 1% PenicillinStreptomycin (Gibco)

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The Centers for Disease Control and Prevention (CDC) reports that approximately 3% of adults over age 50 will develop a serious B12 deficiency that can impair health

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If you suspect a B12 deficiency or have ongoing symptoms, seek appropriate medical evaluation and lab-guided care

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