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Hepatocytes were cultured in DMEM/F12 culture media (Gibco, 21331-020) supplemented with 10% FBS for the first 3 h post isolation, together with 10,000 U/ml penicillinstreptomycin (Gibco, 15140-122), 200 mM l-glutamine (Gibco, 25030-024), 7.5 mM d-glucose (Sigma, G6152), 150 mM Hepes pH 7.4 (Sigma), and 1 mM methionine (Sigma) (except for MCD hepatocytes) that were used as culture medium

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