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Activation methods which are used in affinity chromatography can be summarized as follow: Amine groups : Cyanogen bromide (CNBr) method Schiff base (reductive amination) method N-hydroxysuccinimide (NHS) method Carbonyldiimidazole (CDI) method Cyanuric chloride method Azalactone method (for Emphaze supports) Divinylsulfone (DVS) Epoxy (bisoxirane) method Ethyl dimethylaminopropyl carbodiimide (EDC) method Tresyl chloride/tosyl chloride method Sulfhydryl groups: Azalactone method (for Emphaze supports) Divinylsulfone method Epoxy (bisoxirane) method Iodoacetyl/bromoacetyl method Maleimide method Pyridyl disulde method TNB-thiol method Tresyl chloride/tosyl chloride method Hydroxyl groups: Cyanuric chloride method Divinylsulfone method Epoxy (bisoxirane) method Aldehyde groups Hydrazide method Carboxyl groups Ethyl dimethylaminopropyl carbodiimide (EDC) method [8]

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