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N-acetyl cysteine (a ROS scavenger) and U0126 (a mitogen-activated protein kinase [MAPK] inhibitor) can rescue PFOS-mediated autophagy activation and, consequently, autophagy-associated apoptosis in RTCs 3

In a 48-well plate, 250 l diluted cells (50,000 cells/well) were combined with 125 l of the transfection complex mixture and incubated for 24 h before removing RNP medium and replacing with DMEM with 10% FBS and 100 units/ml penicillin/streptomycin (15140122

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