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Wawilow, 1927) with Kashas rule emphasizing that the emitting level of a given multiplicity is the lowest excited level of that multiplicity (Kasha, 1950) gives rise to an important principle that the shape of the fluorescence spectrum is independent of the excitation wavelength

RNA purity and integrity were determined using a NanoDrop ND-1000 (NanoDrop Technologies) and an Agilent 2100 Bioanalyzer (Agilent Technologies)

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Due to the parallel orientation of both electrons (electron spin S = 1), molecular oxygen is sometimes referred to as triplet oxygen (multiplicity 2 S + 1 = 2 1 + 1 = 3) and is denoted as 3 O 2 (Halliwell and Gutteridge 2015)