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This method involves four main steps: isolation by strong cation-exchange SPE, mild derivatization with pentafluorophenyl trifluoromethanesulfonate, chromatographic separation using sequential ion-exchange/reversed-phase (ultra) high-performance liquid chromatography [(U)HPLC], and detection by electrospray ionization MRM-MS

Surgical intervention involves the treatment of choice when PD has reached its stable phase and in cases presenting with severe erectile dysfunction and/or severe penile curvature preventing complete sexual intercourse, while medical therapy is indicated instead when PD is in its initial, inflammatory phase

elegans , the fat content in daf-2 mutants is significantly increased, and the excess body fat is largely in the form of storage TAGs [75]

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