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Binding to GHS-R1a activates Gq/11-mediated phospholipase C signaling, leading to IP3 and DAG generation, intracellular calcium mobilization, and downstream signaling cascade activation

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Retarutide inhibited human GCGR, GIPR and GLP-1R with EC50 values of 5.79, 0.0643 and 0.775nM, respectively

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If particulates persist after 15 minutes total, the peptide may have aggregated during manufacturing or shipping, or the vial experienced temperature abuse