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[DOI] [PMC free article] [PubMed] [Google Scholar] 336.Moore, M

Med Clin North Am 2008

Detection of HGF and c-Met expression in gastric mucosa by ELISA The gastric mucosa tissues (the ulcer and normal gastric mucosa of around 5 mm, weighing 500 mg) from another five samples in each group were taken and homogenized with a homogenizer in a lysis buffer containing 62.5-mmol ethylenediaminetetraacetic acid, 50-mmol Tris, pH 8.0, 0.4% deoxycholic acid, 1% Nonidet P-40, 0.5-mg/mL leupeptin, 0.5-mg/mL pepstatin, 0.5-mg/mL aprotinin, 0.2-mmol phenylmethylsulfonyl fluoride, and 0.05-mmol aminoethyl benzene sulfonyl fluoride

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