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The extraction process was repeated four times

The column was maintained at 40 C with a constant flow rate of 400 nL/min

Injuries to the CNS often compromise the integrity of the bloodbrain or bloodspinal cord barriers, leading to increased permeability, immune cell infiltration, and the release of inflammatory mediators that exacerbate neurodegeneration
The latter effect was probably due to S-sulfhydation and activation of the autophagy regulator serum and glucocorticoid-regulated kinase 1 (SGK1) at Cys244 and Cys282 induced by NaHS (200 M for 24 h), as demonstrated on cultured HepG2 hepatic cells
Autism 7 , 47 (2016)

For instance, Huang et al
