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The outer skin may be pulled away from the muscle tissue to ensure the needle doesn't pierce the muscle

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102 A placebo-controlled trial that included 54 overweight men with vitamin D deficiency participating in a weight loss program found 3,332 IU (83.3 mcg) of vitamin D daily for one year increased total testosterone (from 10.7 to 13.4 nmol/L [309 to 386 ng/dL]), bioavailable testosterone (from 5.21 to 6.25 nmol/L [150 to 180 ng/dL]), and free testosterone levels (from 0.222 to 0.267 nmol/L [6.4 to 7.7 ng/dL]), whereas placebo led to no significant changes in testosterone levels

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One patient with SRD treated with valproic acid was reported, but no details regarding the clinical course were provided