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The 96-well plates were centrifuged at 30 g , 5 min, room temperature to create loose pellets of yeast cells at the bottom of the wells
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Ubiquitylation of mitochondrial proteins is extensively described for selective autophagic removal of mitochondria through a process called mitophagy [74], however, in recent years, ubiquitylation of cytosol-invading bacteria has also been implicated to induce an innate immune response [75,76,77]
