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Structure of R152H GPX4 is modeled based on PDB structure 6HN3

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5 , e1000361, (2009)

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For acute stimulation experiments, cells were cultured with LPS and IFN- for 2 h, after which stimuli were removed, cells were washed with Dulbeccos PBS (dPBS), and cell culture was continued in fresh media without stimuli for the remaining time course