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Each filtrate was adjusted with the homogenization buffer to make a final volume of 1.5 ml, and then, their fluorescence emission spectra (500600 nm) were recorded on a fluorescence spectrophotometer (FP750, Jasco, Japan) with excitation at 446 nm

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Treatment with LPS combined with the proinflammatory cytokine interferon- (IFN- ) is frequently used to induce and study the M1 type of microglial activation in vitro