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61 StafaK.TsikaE.MoserR.MussoA.GlauserL.JonesA.et al

Every millilitre you draw contains all four peptides at their fixed ratio

Thats it the whole peptide

Adenovirus-Mediated Expression of CYP2E1 Produces Liver Toxicity in Mice

The mean CT values for each sample were used to calculate relative expression using a variation of the 2^(- delta delta CT) method 36

doi: 10.1093/ndt/gfg485