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Its name gives away its primary function: promoting delta-wave sleep, the deepest and most restorative stage of the sleep cycle

Occludin regulates macromolecule flux across the intestinal epithelial tight junction barrier

Funding This work has been supported by R01NS112373 to Dr

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Membranes were sedimented by ultracentrifugation at 150,000 g at 4 C for 1 h, and resuspended in 16 mL of solubilized buffer (1%(w/v) DDM, 150 mM NaCl, 10%(v/v) glycerol, 1 mg mL 1 iodoacetamide, 0.2 mM TCEP, 0.1 protease inhibitor cocktail, 50 mM Tris-HCl pH 8.0)

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