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Description
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AcrA is arranged linearly into four domains, the -helical hairpin, lipoyl, -barrel, and membrane proximal (MP) domains (Figure 2b) 46,5658 According to cross-linking and mutagenesis studies and more recently cryo-EM imaging of the assembled complex, while the -helical hairpin domain interacts with TolC, the other domains interact with AcrB

The simple Mendelian autosomal recessive heredity of the disease was established by the study of the genealogy of the affected cattle and by breeding experiments

(1994a)

[DOI] [PubMed] [Google Scholar] 233.Sarker M.K., Lee J.H., Lee D.H., Chun K.H., Jun H.S

Five positions judged to govern ketosteroid isomerase activity based on mutational studies ( The catalytic efficiency of HsaGST A3-3 with 5-AD is 5000-fold higher than that of HsaGST A2-2, and we found that mutating the five residues in the H-site of HsaGST A2-2 into the corresponding HsaGST A3-3 residues enhanced the efficiency to 70% of the HsaGST A3-3 value (Table 1)
