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Co-immunoprecipitation mass spectrometry and CRISPR KO in cells identified that mitochondrial m -AAA protease AFG3L2 is responsible for degrading SLC25A39 through the matrix loop 1

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Future medicinal chemistry efforts may yield next-generation compounds with enhanced properties, including improved tissue selectivity, modified pharmacokinetics, or additional mechanistic activities that complement NNMT inhibition

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Possibly disturbing factors have been examined to avoid artifact determinations