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Overall, these mechanistic insights uncover a passage-associated shift from biosynthetic activity toward redox maintenance and identify the cysteine-glutathione axis as a critical metabolic lever for enhancing long-term stability and productivity in CHO cell culture.

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The no-NAC group sustained perfusions for an average of 96 (range 87102) h, universally developing methaemoglobinaemia (2%) observed after an average of 45 h, with subsequent steep rise