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Concentrated virus was added to day 2 cultured CD34+ erythroid progenitors in the presence of 8 g/ml polybrene

Subsequently, for these data as well as the alpha diversity analyses, each treatment group (within each phylum or genus for the relative abundance data) was analyzed for normal distribution (ShapiroWilk test) and the data across groups were tested for homoscedasticity (BrownForsythe test)

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Conversely, supplementing Gln restores ZO-1 expression and barrier function, even under stress conditions such as methotrexate or acetaldehyde exposure (Kim and Kim 2017)