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ACTL6A is found to be critical for regulating the glutathione (GSH) metabolism pathway because it upregulates -glutamyl-cysteine ligase catalytic subunit (GCLC) expression, thereby reducing reactive oxygen species (ROS) levels and inhibiting ferroptosis, a regulated form of cell death driven by the accumulation of lipid-based ROS

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Pathway inhibitor studies using specific kinase blockers confirm signalling cascade hierarchy and cross-talk mechanisms