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Cyclic AMP determination To measure intracellular cAMP concentrations, H9c2 cells and NMCMs were pretreated with 3-isobutyl-1-methylxanthine (0.1 mM, IBMX, Sigma-Aldrich) for 30 min and then stimulated with the non-selective MC-R agonist -MSH (0.1 nM) or the MC5-R agonist PG-901 (0.1 nM) for 5, 15, 30, and 60 min
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Inject water slowly down the wall of the vial
(1999) Genetic relationships between selection for growth and reproductive effectiveness
Oktober 1997, fr V
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