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PMID 1885604

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240,241 Disrupting ALDH activity with drugs could result in the accumulation of toxic levels of ROS, leading to DNA damage and apoptosis in a subset of DTP cells

After harvesting the cells by centrifugation at 4,000 g for 30 min, the cells were resuspended in lysis buffer (50 mM sodium phosphate, pH 8.0, 300 mM NaCl, 10 mM imidazole) and lysed using a sonication for 1 h with a 10-s pulse mode (10 s on and 10 s off) on ice

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J Vasc Res (2010) 47(5):42331