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10.1093/ajcn/79.5.727

RNC-NAC-NMT1 in vitro reconstitution for cryo-EM 100 nM RNCs were incubated with 135 nM wildtype NAC and 135 nM GST-NMT1 in Reconstitution Buffer (20 mM HEPES/KOH pH 7.5, 150 mM KOAc, 5 mM MgCl 2 , 1 mM DTT, 0.05% octaethylene glycol monododecyl ether, 100 mM sucrose) for 1 h on ice before directly preparing cryo-EM grids

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