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During electron transfer through the respiratory chain, mitochondria generate large portions of ROS, such as superoxide, hydroxyl radical, hydroperoxyl radical, and hydrogen peroxide (H 2 O 2 ), into the matrix and the intermembrane space

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This included reduced activities of glutathione synthetase (GSS), Glutamate-Cysteine Ligase Catalytic (GCLC), and glutathione S-transferase (GSTS) (Supplementary Figures S7AC)

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Ca 2+ signaling may play an important role in steroid synthesis, the initiation of ovulation-related genes, and the regulation of mitochondrial function