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doi: 10.1111/joim.13592

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It acts as a cofactor for enzymes involved in the metabolism of macronutrients, including carbohydrates, proteins, and fats

Correction of hypothyroidism seems to have no effect on body fat

Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]

[DOI] [PMC free article] [PubMed] [Google Scholar] 147.Jukic A.M.Z., Wilcox A.J., McConnaughey D.R., Weinberg C.R., Steiner A.Z