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As a control for specificity of transfer, the lipid binding domain of a mammalian STARD3 (His-STARD3-StART) was used because this protein was previously shown to interact with sphingosine in related work (51 Preprint ), but it is not located inside lysosomes and, therefore, is not expected to interact with the NTD

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Ascertainment of ASCVD MESA event surveillance and classification have been described 16