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For fixed imaging, worms were first washed three times with 1 mL of 0.1% Tween in 1 PBS, resuspended in 1 mL of 100% methanol, and incubated at 30 C for 5 min

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[DOI] [PMC free article] [PubMed] [Google Scholar] 129.Pawlotsky JM, Tsakiris L, Roudot-Thoraval F, Pellet C, Stuyver L, Duval J, Dhumeaux D

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