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Description

Jenkins et al., 2021

J.GodinoA.SaleryM.NestlerE

Elderly men often suffer from metabolic syndrome, including type 2 diabetes and hyperlipidemia

Materials and methods Strains and culture conditions Yeasts were maintained and grown either in YPD broth [1% yeast extract (Oxoid), 2% bacteriological peptone (Oxoid), 2% D-glucose], or in YNB broth [0.69% yeast nitrogen base without amino acids (Formedium), 2% D-glucose] supplemented as required with amino acids, uracil, 5-FOA (Melford), D-histidine (Sigma) or reduced GSH (Sigma)

Given the limited availability and heterogeneity of this tissue, developing a standardized and unlimited cell source with high efficacy and safety would greatly assist in the commercialization efforts of ESC-based SCTs