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After the plates were treated with 1D228 (10, 100 and 1000 nM) or DMSO (0.32% or 0.64%) for 48 h at 37 C, then cultuerd in 10% FBS medium, the colonies were fixed with 4% paraformaldehyde and stained with 1% crystal violet solution for 20 min

MicroRNA-146a decreases high glucose/thrombin-induced endothelial inflammation by inhibiting NAPDH oxidase 4 expression

Supplements with intrinsic factor: Individuals with pernicious anemia may be advised supplements containing intrinsic factor, a protein facilitating B12 absorption

Once reconstituted, refrigerate at 2-8C and use within 2-4 weeks

The effect of this synergistic treatment is ASS-dependent, and the activity of the enzymes mentioned above can be restored by transfection of ASS, eliminating the sensitivity of tumor cells to ADI-PEG20 combined with 5-FU treatment